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Virtual Naval Hospital

Medical Management of Biological Casualties Handbook

Appendix G: BW Agent Lab Identification

U.S. Army Medical Research Institute of Infectious Diseases,Fort Detrick, Frederick, Maryland
Peer Review Status: Internally Peer Reviewed


Immunoassays

Agent Gold Standard Antigen Capture IgG IgM PCR Animals

Aflatoxins Mass spectroscopy

Alpha Toxin ELISA X

Alphaviruses Virus isolation/neutralization X X X X X

Arboviruses Virus isolation/IFA X X X X X

Bacillus anthracis IFA/Std. Microbiology X (PA) X X X X

Bacillus globigii Std. Microbiology X

Bacillus thuringiensis Std. Microbiology

Bot Toxin Mouse neutralization X (A Toxin) * X

Brucella sp. IFA/Std. Microbiology X X X X X

C. burnetii IFA/Std. Microbiology/serology X X

C. perfringens

Clostridium sp. Std. Microbiology

F. tularensis IFA/Std. Microbiology X X X

____________________________________________________

*Toxin gene detected

ELISA - enzyme-linked immunosorbent assays

IFA - indirect or direct immunofluorescence assays

Std. Micro./serology - standard microbiological techniques available, including electron microscopy

Immunoassays

Agent Gold Standard Antigen Capture IgG IgM PCR Animals

Filoviruses Virus isolation/neutralization X X X X X

Hantaviruses Virus isolation/IFA X X X X X

Orthopox Viruses Virus isolation/IFA X X X

Ricin Toxin ELISA X X X

Saxitoxin Bioassay (neutralizing antibodies) X

SEA Toxin *

SEB Toxin ELISA X * X

Shigella sp. Std. Microbiology

Tetrodotoxins Bioassay (neutralizing antibodies) X

Vibrio cholerae Std. Microbiology/serology X X X X

Yersinia pestis IFA/Std. Microbiology X (F1) X X X X

______

*Toxin gene detected

ELISA - enzyme-linked immunosorbent assays

IFA - indirect or direct immunofluorescence assays

Std. Micro./serology - standard microbiological techniques available, including electron microscopy

 

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